ImmunoProtect™ Hybridoma Sequencing

ImmunoProtect™: Hybridoma Sequencing

Your monoclonal antibody (mAb) is extremely valuable, but hybridoma cell lines can become unstable over time and can stop secreting antibody. With ImmunoProtect™, the DNA sequence of your antibody will be determined and can be used to express your mAb recombinantly should your hybridoma cell line become unstable.

Project Phases	Timeframe
I. DNA Sequencing Isotype/subisotype determination Mycoplasma Testing RNA Isolation, cDNA preparation, PCR amplification DNA sequencing of antibody heavy and light chain variable regions Phase I Deliverables DNA Sequence of antibody heavy and light chain variable regions Sequence Analysis Report Mycoplasma Non-Contamination Certificate	2 Weeks
II. Recombinant Antibody Binding Confirmation and Test Expression Cloning of heavy and light chain variable regions into mammalian expression system Transient test expression of antibody in mammalian cell line to determine expression levels Confirmation of antigen binding by indirect ELISA Phase II Deliverables 40mL of tissue culture supernatant containing expressed antibody Report on antigen binding and expression level of the antibody	3-4 Weeks

Project Phases

Timeframe

I. DNA Sequencing

Isotype/subisotype determination
Mycoplasma Testing
RNA Isolation, cDNA preparation, PCR amplification
DNA sequencing of antibody heavy and light chain variable regions

Phase I Deliverables

DNA Sequence of antibody heavy and light chain variable regions
Sequence Analysis Report
Mycoplasma Non-Contamination Certificate

2 Weeks

II. Recombinant Antibody Binding Confirmation and Test Expression

Cloning of heavy and light chain variable regions into mammalian expression system
Transient test expression of antibody in mammalian cell line to determine expression levels
Confirmation of antigen binding by indirect ELISA

Phase II Deliverables

40mL of tissue culture supernatant containing expressed antibody
Report on antigen binding and expression level of the antibody

3-4 Weeks

As with all our services, we will tailor an antibody sequencing package for your specific needs. Contact us to discuss your hybridoma health, stability or antibody secretion challenges.