IPA offers a selection of purification procedures for isolation of specific antibody from serum, ascites fluid, and concentrated tissue culture supernatant. All purified products undergo a full round of analysis to ensure high quality antibody has been generated. These purified products are ready for use in your important experiments or downstream manufactured products.
Protein A/G Chromatography
High yields of pure IgG can be obtained using Protein A or Protein G chromatography, which differ in their ability to bind antibodies of different origins and subclasses. Both are rapid, reproducible and amendable to scale-up, and are effective in aseptic purification strategies.
|Materials needed from client:||Ascites fluid, tissue culture supernatant or hybridoma clone for culture.|
|Total project time:||Depends on starting material. Approximately 2 week timeline for purification, analysis and shipment preparation.|
|Yield:||Dependent on starting material and purification method.|
|Mouse ascites fluid||Protein G|
|Rat ascites fluid||Protein G|
|Mouse bioreactor tissue culture supernatant||Protein G|
|Rabbit monoclonal||Protein G|
|Raw rabbit serum||Protein A|
|Raw goat or sheep sera||Protein A|
ImmunoPrecise offers affinity purification of anti-sera. Our capabilities include customized peptide affinity and KLH depletion columns.
Contact us for more information about this service.